- Our TZ node won a prize at the World Government Summit Awards in Dubai UAE for applying Nanopore sequencing for smallholder farmers – Congrats. The team was on Tanzanian morning TV.
- We successfully trained scientists at University in Eldoret and JKUAT on bioinformatics and running the MINion. Many thanks for the Crawford Fund for supporting the work and also the 69 people who donated to our crowdfunding page! Please see Professor Elizabeth Njenga and Dr. Jayne Binot describing the course and what it means to the team in Kenya [sorry about the wind, I’m not a video pro]
- And we are excited to be invited to give a talk at the London Calling Meeting in May! We will see you all there!
- Special videos to our crowdfunding supporters: (we promised we would shout their names form the mountain in Kenya) .
A very challenging two weeks but we did it, we made it. We have data for 12 cassava plants – some showing symptoms and some were not.
Dr. Naomi Bissem’s tweet says it all:
Details: We learned A LOT on this trip. Shipping was a challenge. We discovered FedEx froze our flow cells- and this is no joke. It ruined all the flow cells but luckily Nanopore sent us new flow cells (as per the suggestions below) via DHL. We learned that that little gray pad on the flow cell is super important for temperature control (thank you twitter for helping real-time). We learned that the priming of the flow cell is important and if your library isn’t going in the spot-on port to add more priming mix to the flow cell to get things moving. We learned that power is not a given. We should have had a battery back up on the machine we were using. We also learned once (actually 3 times) when the power goes off you can quickly start the machine running and it keeps sequencing.
The team: I also learned the team from University of Eldoret has some serious tenacity. They boarded a bus Thursday night upon hearing we received the shipment- drove 6 hours- and we began preparing the library Friday morning, started the run (3 times with the power cuts) and then got back on the bus and drove back to Eldoret. In addition, Charles Kayuki, from Mikocheni Agricultural Research Institute (MARI) joined us for the second week at JKUAT – he was a valuable resource as he has completed runs on his own in Tanzania. Anders Savill in Australia was on-call and dealing with us at all hours- thank you Anders. Plus Angelica and Zoe from Nanopore were amazing at facilitating so many things on that end. Finally, the JKUAT team lead by Professor Ateka- was invaluable, fierce, dedicated and downright amazing. Elijah Ateka, Brenda Muga, Sam Maura, and Benson – Asante Sana for relentless fight to get this test done.
The farmers: Rose, from Eldoret had CMD symptomatic plants. We tested her plants and Prof. Ateka provided her the virus resistant cassava. The team from Eldoret took the cuttings to her and she is on her way. We also visited two other farms, Mama Maina and Mr. Lungo Mutunga’s farm in Thika, Kenya. We are awaiting the results of the run and will advise them later this week.
Prison Visit: While we waited for the flow cells to arrive we visited Langata Women Maximun Security Prison to conduct a cassava pest and disease training for inmates and farm managers. We feel very strongly empowering all farmers is at the core of our movement. This was a very special day and the team plans to go back and plant virus-free cassava with the inmates on the prison farm. Outreach matters.
Ok, so we had ZERO pores on our two flow cells. We suspect our flow cells were frozen in customs. Why? 1) It’s the first time they have been shipped here (so these items were not in the Kenyan customs officials database) and 2) We feel the labeling on the Nanopore box needs a bit of improvement when shipped to Kenya (and E. Africa in general). So here are our suggestions:
1. Ship separate boxes for each temperature (customs can’t open the box to separate).
2. In large font on the box “Store Freeze -20C” OR
3. In large font on the box “Store Fridge 2-8C”
4. Highlight the temperature in the paperwork
Paperwork taped ON THE BOX:
1. Clearly state the material is not poisonous or for human consumption
2. Clearly state it has no resale value and is for research only
Elijah is a star!!! He freed the box from customs. And now we are on our way. We visited Rose – a farmer here in Eldoret, Kenya she has a small plot of cassava and we suspect it is infected soon we will know!
The team here at University of Eldoret has kindly hosted us in their biotechnology lab for the DNA extractions.
Tomorrow we do the library prep & start the nanopore run!
Our things have been stuck in customs for 2 weeks. Elijah has driven 5 hours one way (back to Nairobi from Eldoret) and is planning a sit-in at the customs building to get our supplies. Then he and Benson are driving another 5 hours back to Eldoret! The shipping struggle is real. If anyone has connections, solutions, ideas about making this easier please get in touch. We are determined to do this on Thursday with the class at University of Eldoret. Wish us luck.
2 weeks left before we leave. The shipment from Nanopore is on it’s way to Prof. Ateka in Kenya. We have ordered two of the R9.4 flow cells – to go with the 1 new Rapid Barcoding Kit RBK004. It was advised to use the 9.4 flow cells instead of the 9.5 so that is what we will do! Also the Anders Savill has been preparing the MacMini we are taking with us to do the work and then leave at JKUAT. The third time around things are falling into place. It’s all very exciting.